Total-Body Potassium in Rats Determined by Compact in Vivo Neutron Activation Analysis Assembly
S Tabbassum1*, C Pinjing2, F Yanko3, R Balachandran4, M Aschner5, A Bowman6, H Nie7, (1) PURDUE UNIVERSITY, W Lafayette, IN, (2) PURDUE UNIVERSITY, W Lafayette, IN(3) PURDUE UNIVERSITY, W Lafayette, IN(4) PURDUE UNIVERSITY, W Lafayette, IN(5)Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, ,,,(6) PURDUE UNIVERSITY, W Lafayette, IN(7) Purdue Univ, West Lafayette, IN
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PO-GePV-M-202 (Sunday, 7/25/2021) [Eastern Time (GMT-4)]
Purpose: This study aimed to evaluate the feasibility of a compact neutron generator-based in-vivo measurement system to quantify total body potassium in small animals using rat carcasses.
Methods: A cohort of thirty-nine rats (n= 20 males and 19 females, average weight 255±15 and 163±7 gram), which were part of a manganese exposure study, were sacrificed and inserted in polyethylene bottles. These rats were then placed and irradiated in a carefully designed irradiation cave built beside the neutron generator with an optimized thermal neutron flux and radiation dose ratio. The irradiation time was 10 minutes, followed by a 10-minute decay and 2-hour measurement using the high purity germanium detector
Results: The average K concentration in male and female rats was significantly different (3546+/-473 vs. 2258+/-281μg/g). There was a significant correlation between potassium concentration and weight in both male and female groups (for male r(20) =.48, P=0.03, for female r(19)=.59, P=.007). We evaluated the impact of manganese exposure on potassium, and potassium concentration was not affected by manganese exposure
Conclusion: This work shows that in-vivo neutron activation analysis can be a potential way to explore in-vivo potassium storage and metabolism.
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