Effect of High-Dose-Rate Irradiation On Cell Survival:Simple Investigation of Cultured Cells
M Kurimoto1*, K Yasui2, T Watanabe3, H Yanagi4, Y Saito5, S Ogawa5, N Hayashi2,S Hayashi6, (1)Graduate School of Health Sciences, Fujita Health University. (2)School of Medical Sciences, Fujita Health University. (3)Cancer Center Division of Gene Regulation, Fujita Health University. (4)Department of Medical Oncology, Fujita Health University School of Medicine. (5)Division of Radiology, Fujita Health University Hospital. (6) Department of Radiation Oncology, Fujita Health University School of Medicine.
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PO-GePV-T-441 (Sunday, 7/10/2022) [Eastern Time (GMT-4)]
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Purpose: High-dose-rate irradiation contributes to decreasing radiation therapy time and reducing organ displacement due to respiratory motion. It is known that radiation dose rate impacts cell survival. However, detailed evidence of dose-rate effects is insufficient because an experimental system using cultured cells has not been unified in previous studies. The purpose of this study was to establish a suitable experimental system for culturing cells using a linear accelerator and to verify the effects of high-dose-rate irradiation on these cultured cells.
Methods: Kidney-derived non-cancer (HEK293 and HK2) and cancer (Caki-1) cells were irradiated with 10 MV flattening filter-free beams to verify the dose-rate effect. The prescription dose to the cells was set to 2 Gy with four dose rates: 3.83 Gy/min, 23.01 Gy/min, 31.85 Gy/min, and 45.56 Gy/min. The cell survival rate was evaluated using clonogenic and cell proliferation assays.
Results: In the clonogenic assay, the survival rate of Caki-1 cells at 45.46 Gy/min was significantly decreased compared with those at 23.01 Gy/min and 31.85 Gy/min (Student’s t-test, P < 0.05). The survival rates of HEK293 cells at 31.85 Gy/min and 45.46 Gy/min were significantly higher than that at 23.01 Gy/min (Student’s t-test, P < 0.05). In the cell proliferation assay, Caki-1 and HEK293 cell growth showed a trend similar to that in the clonogenic assay.
Conclusion: We established a method by which isolated cultured cells were irradiated using a linear accelerator. Differences in survival rates of both cancer and non-cancer cells were dependent on the radiation dose rate. However, the results of this study showed a trend different from those of previous studies using other cell cultures. There was also large variation among experiments. It is necessary to conduct further studies using dose variation and elucidate the mechanism underlying cultured-cell survival under high-dose-rate irradiation.
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