Nanoparticles Enhance Radiosensitivity and Improve Chemoradiotherapy for Brain Tumors
A Hubbard*, O Salas, E Jank, A Benoit, D Jordan, Y Walter, C Thiegs, A Ekpenyong, Creighton University, Omaha, NE, USA.
Presentations
PO-GePV-T-437 (Sunday, 7/10/2022) [Eastern Time (GMT-4)]
ePoster Forums
Purpose: Glioblastoma remains the most malignant and most common primary brain tumor in adults, with a median overall survival of only 15 months. This bleak outcome is partly due to the high chemoresistance and high radioresistance of glioblastoma. Current standard of care includes surgical resection, chemotherapy and temozolomide (TMZ) as adjuvant therapy. The purpose of this work is to improve treatment outcomes for highly radioresistant and chemoresistant cancers employing nanoparticle mediated radiosensitization and chemoradiotherapy.
Methods: We are using nanoparticle (NP) spectroscopy with a broad range of novel biocompatible nanoparticles including carbon quantum dots (CQD), graphene quantum dots (GQD), and CdSe/Zns quantum dots (QD) based on our recently developed assay involving fluorescence intensity modulation of QDs to assess reactive oxygen species (ROS) generation during chemotherapy and radiotherapy. Our strategy here is to use the NPs for concurrent measurement of ROS and radiosensitization, while applying chemoradiotherapy. We also have a focus on clonogenic assays for cell lines treated with this same broad range of quantum dots along with TMZ for measurement of cell survival two- and three-weeks posttreatment. Using a standard laboratory cell irradiator (Faxitron), we irradiate glioblastoma cancer cell lines (T98G and U87 cells) treated with QDs, CQDs, and GQDs as well as chemotherapeutic drugs such as TMZ.
Results: Cell survival curves from clonogenic assays show improved cell killing following chemoradiotherapy combined with GQDs, compared to the combination with CQDs. More recent results will be presented.
Conclusion: Overall, we have evidence that concurrent radiosensitization using GQDs may lead to improved chemoradiotherapy against glioblastoma cells in vitro.
Keywords
Not Applicable / None Entered.
Taxonomy
TH- Radiobiology(RBio)/Biology(Bio): RBio- general
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